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Blend Taq® & Blend Taq® -Plus-

 เพิ่มเมื่อ: 23/12/2016

รายละเอียด:
High Efficient Taq DNA polymerase
  • Catalog No.TYB-BTQ-101 BTQ-201
  • DescriptionBlend Taq® and Blend Taq® -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns' method(1). This method uses a DNA polymerase which lacked 3'→5' exonuclease (proofreading) activity (e.g., Taq DNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a 'mixed' enzyme solution enables highly efficient amplification. The enzyme solution of Blend Taq® -Plus- contains anti-Taq DNA polymerase antibodies that inhibit polymerase activity, allowing for Hot Start PCR. Blend Taq® and Blend Taq® -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.
  • Feature1 Effective for the amplification of various targets from small starting template amounts. 2 The use of Hot Start technology with anti-Taq DNA polymerase antibodies results in highly efficient amplification. 3 The PCR error ratio of this enzyme is approximately 3-4 times less than that of Taq DNA polymerase.
  • Volumn Unit
  • Application
  • CompanyTOYOBO
  • ManualBlend Taq® or Blend Taq® -Plus-
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Product
Blend Taq® & Blend Taq® -Plus-
Catalog No.
TYB-BTQ-101 BTQ-201
Description
Blend Taq® and Blend Taq® -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns' method(1). This method uses a DNA polymerase which lacked 3'→5' exonuclease (proofreading) activity (e.g., Taq DNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a 'mixed' enzyme solution enables highly efficient amplification. The enzyme solution of Blend Taq® -Plus- contains anti-Taq DNA polymerase antibodies that inhibit polymerase activity, allowing for Hot Start PCR. Blend Taq® and Blend Taq® -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.
Feature
1 Effective for the amplification of various targets from small starting template amounts.
2 The use of Hot Start technology with anti-Taq DNA polymerase antibodies results in highly efficient amplification.  
3 The PCR error ratio of this enzyme is approximately 3-4 times less than that of Taq DNA polymerase.
Volumn Unit and Reaction

                                            
Application
Company
TOYOBO
Manual

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