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Fragment Analysis

วันที่: 10-08-2017

Fragment Analysis & Short Tandem Repeat (STR) Analysis

Amplification reactions are performed by labeling one end of a PCR fragment with a fluorescent taq. This is achieved by using end labeled primer in the PCR reaction. As the fluorescent DNA fragment passes the point of detection on the ABI3730xl sequencer,signals are sent to a macintosh and collected by ABI's Gene Scanner Data Collection software. In minutes the Gene Scanner Analysis software processes the gel image and interprets the data into a colored densitometric scan.internal size standards run in each lane so that the software can automatically calculate the size of each fragment.

Sample Requirements

  • Fluorescent PCR products as a 20 ul volume of your reactions.

  • Send samples in 1.5 ml microfuge tubes or plates

Supported Dye Sets