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NucleoSpin® miRNA

 เพิ่มเมื่อ: 2016-12-01 10:46:45.0

รายละเอียด:
MicroRNA
  • FeaturesParallel isolation of small and large RNARNA purification fractionated by size: Isolation of small RNA only (< 200 b), Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions Isolation of total RNA (small and large RNA in one fraction) • Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis • Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending) • NucleoSpin® Filters for efficient sample homogenization • rDNase for efficient on-column removal of genomic DNA
  • Catalog No.740971.10 / .50 / .250
  • Quantity10, 50, or 250 preps
  • MethodSilica-membrane Mini spin columns
  • Sample material< 107 cells, < 30 mg human / animal tissue, < 50 mg plant tissue, < 150 μL reaction mixture
  • Typical yield10 μg small RNA, 95 μg large RNA (107 HeLa cells)
  • Elution volume30–100 μL
  • Prep time45 min/6 preps (small and large RNA), 35 min/6 preps (small RNA only)
  • CompanyMACHEREY-NAGEL
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Features

Parallel isolation of small and large RNARNA purification fractionated by size: 
   Isolation of small RNA only (< 200 b),
   Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
   Isolation of total RNA (small and large RNA in one fraction)
• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
• Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform 
   (patent pending)
• NucleoSpin® Filters for efficient sample homogenization
• rDNase for efficient on-column removal of genomic DNA
Catalog No.
740971.10 / .50 / .250
Quantity
10, 50, or 250 preps
Method
Silica-membrane Mini spin columns
Sample material
< 107 cells, < 30 mg human / animal tissue,
< 50 mg plant tissue, < 150 μL reaction mixture
Typical yield (µg)
Recovery (%)
10 μg small RNA, 95 μg large RNA (107 HeLa cells)
Elution volume
30–100 μL
Prep time
45 min/6 preps (small and large RNA), 35 min/6 preps (small RNA only)
Company
MACHEREY-NAGEL

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